FIGURE 2.

NgR was responsible for the effects of Nogo-P4 on microglia expressing proinflammatory mediators. Before they were added to the protein-coated wells, microglia were pretreated with NEP1–40 (10 μm) or PI-PLC (0.3 units/ml) for 30 min to interrupt the function of NgR. The cells were then exposed to PBS or Nogo-P4 for 12 h. The proportions of iNOS (A and B), pro-IL-1β (C and D), and COX-2 (C and E) were determined by Western blot. Values are reported as mean ± S.D. (error bars), as a percentage of values determined in the PBS group (control, 100%). The release of NO was assayed by the Griess method (F). An ELISA was performed to detect the production of PGE₂ (G) and IL-1β (H) in the supernatant. Values are reported as mean ± S.D. All data represent the results of three independent experiments. Statistical analysis was performed using Student's t test. *, p < 0.05; **, p < 0.01.