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. 2015 Oct 5;290(48):28915–28931. doi: 10.1074/jbc.M115.668244

FIGURE 2.

FIGURE 2.

PREX2 is dephosphorylated by PP1α and PP2A. A, Western blot analysis of GST or GST PP1α pulldowns from HEK293 cells expressing either V5 PREX2 WT or 1085/87A. EV, empty vector. B, Western blot analysis of HEK293 cells expressing V5 PREX2 WT or 1085/87A with or without co-expression of V5 PP1α. Cells were starved and then treated with DMSO or 100 nm calyculin A, followed by treatment with insulin. C, ability of GST PP1α to dephosphorylate V5 PREX2 isolated from HEK293 cells was assessed and analyzed by Western blot. D, Western blot analysis of starved V5 PREX2 WT or 1085/87A-expressing HEK293 cells that were treated with the indicated concentrations of okadaic acid for 30 min. E, ability of purified PP2A to dephosphorylate V5 PREX2 isolated from HEK293 cells was assessed and analyzed by Western blot. F, Western blot analysis of HEK293 cells expressing V5 PREX2 with or without co-expression of V5 PP1α or HA PP2A (catalytic subunit). Cells were starved and then treated with insulin.