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. 2015 Oct 5;290(48):28915–28931. doi: 10.1074/jbc.M115.668244

FIGURE 4.

FIGURE 4.

Gβγ stimulates phosphorylation of PREX2. A, Western blot analysis of starved HEK293 cells where V5 PREX2 was expressed with or without FLAG/HA Gβγ. B, Western blot analysis of HEK293 cells where V5 PREX2 was expressed with or without FLAG/HA Gβγ. Cells were then starved and treated with DMSO or 500 nm GDC0941 followed by treatment with insulin. C, Western blot analysis of HEK293 cells where V5 PREX2 was expressed with or without FLAG/HA Gβγ. Cells were then starved and treated with DMSO or either 500 nm or 5 μm BEZ235 followed by treatment with insulin. D, Western blot analysis of starved HEK293 cells expressing V5 PREX2 with FLAG/HA Gβγ and V5 PP1α.