TABLE 1.

cDNA encoding AQP4 Δ6Cys mutant was subjected to oligonucleotide-mediated, site-directed mutagenesis, creating a series of mutants in which single cysteines were incorporated into the peptide

Residue numbers refer to the amino acid numbers as reported in previous studies of AQP4 (36).

Residue No.	Amino acid change	Codon change
9	Thr → Cys	ACT → TGC
40	Ser → Cys	TCA → TGC
68	Gly → Cys	GGC → TGC
121	Gly → Cys	GGA → TGC
161	Thr → Cys	ACT → TGC
195	Ser → Cys	TCC → TGC
240	Leu → Cys	CTA → TGC