TABLE 1.
Calculated IC50 values of NIBR3049, ruxolitinib, and tofacitinib in autonomous Ba/F3 cells
The table shows the calculated IC50 values (means ± S.E.) of autonomous Ba/F3 cells stably transduced with ALL-associated JAK3 mutants (V674A and L857P) or double mutant (L857P/Y100A), treated with ruxolitinib, NIBR3049, and tofacitinib. The IC50 mean and S.E. values were calculated as means of triplicate cultures from three different experiments.
| IC50 |
|||
|---|---|---|---|
| JAK1/2 inhibitor Ruxolitinib | JAK3 inhibitor NIBR3049 | JAK1/3 inhibitor Tofacitinib | |
| μm | |||
| Ba/F3 JAK3V674A | 0.1 ± 0.03 | 3.42 ± 0.22 | 0.17 ± 0.05 |
| Ba/F3 JAK3L857P | 1.26 ± 0.2 | 1.15 ± 0.21 | 0.32 ± 0.03 |
| Ba/F3 JAK3L857P/Y100A | 2.33 ± 0.21 | 1.31 ± 0.24 | 0.44 ± 0.05 |