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. 2015 Oct 14;290(48):29035–29044. doi: 10.1074/jbc.M115.682310

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — A2E accumulation and retinal inflammation is not affected by the loss of Atg7. A, A2E accumulation was measured by HPLC in 6-month-old Abca4^−/−Atg7^flox/flox;cre+, Abca4^−/−Atg7^flox/flox;cre-, Abca4^−/−, Atg7^flox/flox;cre+, Atg7^flox/flox;cre-, and WT mouse eyes, n ≥ 6 animals per group. Error bars indicate the mean ± S.D. B, representative immunoblots demonstrating that the presence of A2E in the RPE did not alter the expression of Atg7 in 6-month-old Abca4^−/− as compared with WT mice. C, rate of dark adaptation was monitored by measuring a-wave recovery by the ERG. Abca4^−/−Atg7^flox/flox;cre+, Abca4^−/−Atg7^flox/flox;cre-, Abca4^−/−, Atg7^flox/flox;cre+, Atg7^flox/flox;cre-, and WT mice at 3 months of age were used. n = 3 per group. Error bars indicate the mean ± S.D. D, qRT-PCR analysis was performed to measure the expression of Cfh normalized to Gapdh in 6-month-old Abca4^−/−Atg7^flox/flox;cre+, Abca4^−/−Atg7^flox/flox;cre-, and WT whole eye lysates. Expression in Abca4^−/−Atg7^flox/flox;cre+ and Abca4^−/−Atg7^flox/flox;cre- samples was normalized to the WT value, *, p < 0.001, n = 3 animals per group. Error bars indicate the mean ± S.D. E, immunohistochemistry of Gfap in retinal sections from 6-month-old Abca4^−/−Atg7^flox/flox;cre+ and 6-month-old Abca4^−/−Atg7^flox/flox;cre- mice. Scale bar = 50 μm.