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Trisaccharides generated from heparin by heparin lyase digestion. A, composition of what a fully sulfated heparin molecule would be and of the trisaccharide and Δ-disaccharides that would result from digestion with a bacterial endoeliminase heparin lyase. B, FACE gel analyses of the heparin lyase digest of heparin (Hep Digest) and of the trisaccharides (Hep Digest/Tri) after removing the Δ-disaccharides. Tri** corresponds to an undersulafted trisaccharide.
