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. 2015 Sep 16;290(48):29045–29050. doi: 10.1074/jbc.M115.677401

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Analysis of heparin tetrasaccharide and trisaccharide. A, composition of the fully sulfated Δ-tetrasaccharide (Hep-Tetra) and the trisaccharide (Hep-Tri) after removing the Δ-hexuronate using Hg²⁺ treatment. Residual Hg²⁺ was then removed from the trisaccharide product through cation exchange. B, FACE analyses of the Δ-tetrasaccharide (tetra) and the disaccharide (di) prepared through heparin lyase digestion of heparin, after removal of the Δ-hexuronate(2S) residue, yielding the X1 fragment, and the trisaccharide (tri) and monosaccharide (mono), respectively.