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. 2015 Sep 16;290(48):29045–29050. doi: 10.1074/jbc.M115.677401

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effects of heparin tetrasaccharide and trisaccharide on hyaluronan synthesis and monocyte adhesion. A, representative micrographs showing U937 monocytes adhering to the hyaluronan (HA) matrices of cultures at 4 °C 72 h after stimulation to divide in high glucose (25 mm) alone (Control), or with 0. 018 μg/ml of the trisaccharide (Hep-Tri) or with 0.022 μg/ml of the Δ-tetrasaccharide (Hep-Tetra). The ratios of hyaluronan contents for the Hep-Tri and Hep-Tetra cultures to the average value in the control cultures are indicated. B, the numbers of U937 monocytes bound to the cultures as described in A. The S.D. values are shown for the results of 3 independent cultures for each bar with statistical significance (***, p < 0.05) indicated for the increase in the Hep-Tri-treated cultures as compared with the control cultures.