TABLE 2.
Initial rates based Michaelis-Menten kinetics parameter values on different chitin substrates
| Enzyme |
pVmax/E0 (s−1)a |
pKm (mg ml−1)a |
||||
|---|---|---|---|---|---|---|
| CNWs | α-Chitin | Amorphous chitin | CNWs | α-Chitin | Amorphous chitin | |
| ChiAWT | 11.7 ± 0.9 | 7.6 ± 1.8 | 2.1 ± 0.2 | 0.34 ± 0.07 | 1.5 ± 0.6 | 0.31 ± 0.28 |
| W275A | 4.9 ± 0.7 | 7.5 ± 2.1 | 12.3 ± 0.9 | 0.55 ± 0.20 | 1.8 ± 0.5 | 0.83 ± 0.04 |
| W167A | 5.2 ± 0.8 | 8.9 ± 0.9 | 10.7 ± 1.4 | 0.90 ± 0.52 | 2.3 ± 0.8 | 1.14 ± 0.18 |
a pVmax and pKm values were found by non-linear regression analysis of pvss versus S0 curves according to Equation 3. Standard deviations are from three independent measurements.