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. 2015 Oct 1;290(48):29106–29119. doi: 10.1074/jbc.M115.691311

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Surface plasmon resonance analysis of the binding of TCRs C25 and C7 to NLV·HLA-A2. A, TCR C25 at concentrations of 83.8, 41.9, 21.0, 10.5, 5.3, 2.7, 1.4, 0.7, 0.4, and 0.2 μm was injected over immobilized NLV·HLA-A2 (1000 resonance units (RU)). B, fitting curve for equilibrium binding that resulted in a K_D of 4.7 μm for the C25·NLV·HLA-A2 interaction. C, TCR C7 at concentrations of 106.8, 53.4, 13.4, 6.7, 3.4, 1.7, 0.9, and 0.5 μm was injected over immobilized NLV·HLA-A2 (1000 resonance units). D, fitting curve for equilibrium binding that gave a K_D of 5.1 μm for the C7·NLV·HLA-A2 interaction.