. 2015 Jan 31;3:175–188. doi: 10.1016/j.bbacli.2015.01.005

Table 1.

Direct measurement of HDL-C mass by precipitation.

Plasma or serum HDL-C concentration is commonly determined by precipitation methods using various reagents. Reagents involve polyanions such as heparin, dextran sulfate, and sodium phosphotungstate, which are used with a divalent cation, such as magnesium, heparin–manganese, or calcium.

Precipitation
• Polyanions: heparin–Mn²+, dextran sulfate–Mg^2 +[32]
• Divalent cation: dextran sulfate–Mg^2 +[34]
• Polyethylene glycol [37], [44], [126], [197]
• Immunoprecipitation [33]