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. 2015 Nov 27;472(Pt 3):379–391. doi: 10.1042/BJ20150147

Figure 3. Functional analysis, localization and purification of PSKR1–GFP.

Figure 3

(A) Phenotypes of 5-day-old seedlings from wild-type and 35S:PSKR1-GFP expressed in the wild-type background. (B) Mean±S.E.M. root lengths were determined in two independent experiments with at least 46 seedlings analysed per genotype. The asterisk indicates significantly different values between wild-type and 35S:PSKR1-GFP (P<0.05, two-sample Student's t test). (C) PSKR1–GFP localization was analysed by confocal laser-scanning microscopy in mesophyll protoplasts of 4-week-old soil-grown 35S:PSKR1-GFP plants. The receptor protein is expressed and localized at the plasma membrane. Scale bars, 5 μm. (D) Immunoprecipitation of PSKR1–GFP from total protein extracts obtained from seedlings expressing PSKR1–GFP with anti-GFP antibody added at the concentrations indicated. As a control, extract from wild-type seedlings was analysed. The proteins immunoprecipitated from wild-type and PSKR1–GFP extracts were separated by SDS/PAGE (10% gel) and fusion protein was detected with an anti-GFP antibody. Molecular masses of marker proteins are indicated in kDa. Ab, antibody; IP, immunoprecipitation; WB, Western blot; wt, wild-type.