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. 2015 Nov 27;472(Pt 3):367–377. doi: 10.1042/BJ20150660

Figure 4. Knockdown of HDAC3 increases the levels of acetylated tubulin.

Figure 4

(A) Representative blots for acetylated tubulin (Ac-tubulin), α-tubulin, HDAC6 and HDAC3, and the loading control (ERK) for HDAC3 siRNA KD cells, HDAC6 siRNA KD cells, cells treated with non-targeting RNA (NT) or cells left untreated (UT). (B–E) Quantification of HDAC3 levels (B), HDAC6 levels (C), acetylated tubulin levels (D) and α-tubulin levels (E) for HDAC3 KD, HDAC6 KD, NT and UT cells. (F) Representative images of cells stained for actin (red in merged image), acetylated tubulin (green in merged image) and α-tubulin (blue in merged image) in UT, HDAC3 KD and HDAC6 KD cells. Means±S.E.M. are shown. *P< 0.05.