Skip to main content
. 2015 Nov 27;472(Pt 3):367–377. doi: 10.1042/BJ20150660

Figure 5. Overexpression of HDAC3 reduces levels of tubulin acetylation.

Figure 5

(A) Cells expressing eGFP–HDAC3, co-stained for tubulin and acetylated tubulin. Arrows indicate regions where there is some co-localization between eGFP–HDAC3 and acetylated tubulin. (B) Cells stained for acetylated tubulin (Ac-tubulin) in HDAC3 KD cells, control [untreated (UT) cells] and in cells expressing eGFP–HDAC3 (as in A). (C and D) Changes in levels of acetylated tubulin assessed from fluorescent images for eGFP–HDAC3 expression and HDAC3 KD experiments. (E) Assessment of total tubulin expression from fluorescent images for KD experiments. n=9 cells, data shown as means ± S.D. ***P< 0.001.