Figure 6. Effect of HDAC3 KD and expression of GFP–HDAC3 on mitosis.

(A) Representative images of mitotic cells for controls [treated with non-targeting (NT) siRNA], HDAC3 KD and GFP–HDAC3-expressing cells. The control and HDAC3 KD cells were stained for acetylated tubulin (red), with DAPI (blue) and for F-actin (green). Two examples are provided for HDAC3 KD cells to demonstrate that we did see some collapsed spindles as reported previously [27]. GFP–HDAC3 cells were only stained for acetylated tubulin (red) and DAPI (blue), and the image for GFP–HDAC3 is shown in green. (B) Quantification of spindle and chromosome areas for control, HDAC3 KD and GFP–HDAC3-expressing cells. Ten to twelve cells were imaged at high magnification and areas were quantified in imageJ. Individual results are shown with means ± S.E.M. overlaid.