Figure 7. Effect of active HDAC3–SMRT–DAD complex on tubulin acetylation and tubulin association in vitro.

(A) The HDAC3–SMRT 350–480 complex is enzymatically active and can be readily inhibited by the HDAC inhibitor SAHA. (B) Representative Western blots for acetylated tubulin, and for β-tubulin (as a control for total amount of tubulin) in the absence (Control) and presence of the HDAC3 complex following incubation for different lengths of time. (C). Representative Western blot for a microtubule-binding assay at two different HDAC3 complex concentrations, in the presence or absence of microtubules.