. 2015 Nov 27;5:17356. doi: 10.1038/srep17356

Table 3. Results of kinetic assays for E. coli WT and mutant TS enzymes.

	dUMP			mTHF
	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(s⁻¹μM⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m(s⁻¹μM⁻¹)
WT^¶	0.32 ± 0.02	82 ± 9	0.004 ± 0.0005	0.28 ± 0.01	77 ± 8	0.004 ± 0.0004
WT^║	0.60 ± 0.33	58 ± 9	0.01 ± 0.002	0.48 ± 0.04	59 ± 14	0.008 ± 0.002
Ratio^**	mutant/WT	mutant/WT	mutant/WT	mutant/WT	mutant/WT	mutant/WT
G23K^¶	0.94 ± 0.07	0.37 ± 0.07	2.56 ± 0.54	0.86 ± 0.08	0.64 ± 0.15	1.35 ± 0.33
E82K^¶	0.16 ± 0.03	1.10 ± 0.44	0.14 ± 0.06	0.11 ± 0.04	1.19 ± 0.81	0.09 ± 0.07
E86K^║	2.17 ± 0.27	2.41 ± 0.80	0.90 ± 0.32	1.67 ± 0.40	2.54 ± 1.32	0.66 ± 0.37
G257K^║	0.57 ± 0.06	0.86 ± 0.23	0.66 ± 0.19	1.10 ± 0.19	2.71 ± 1.09	0.41 ± 0.18
E. coli_2K^║	1.83 ± 0.44	1.86 ± 1.09	0.98 ± 0.62	1.88 ± 0.56	2.37 ± 1.61	0.79 ± 0.59
E. coli_4K^¶	0.25 ± 0.06	~0.1	~1	0.21 ± 0.04	~0.2	~1

^¶and ^║ refer to independent sets of experiments and comparison within that set. See Fig. S4 for measured kinetic activities. Only approximate K_m values could be obtained for the E. coli_4K mutant as they fell outside the measurable range.

^**Standard error for the ratio Inline graphic