Table 2. Heterologous expression of bmi genes in burkhocin-susceptible strains.
| Indicator strain | Immunity gene | BurM1 (MEX-5) | BurM2 (AMMD) |
|---|---|---|---|
| LMG 18830 | Control | + | ─ |
| bmiAMEX-5 | ─ | ─ | |
| bmiBAMMD | + | ─ | |
| LMG 18941 | Control | + | T |
| bmiAMEX-5 | ─ | T | |
| bmiBAMMD | + | ─ | |
| LMG 18943 | Control | + | T |
| bmiAMEX-5 | ─ | T | |
| bmiBAMMD | + | ─ | |
| LMG 24506 | Control | + | ─ |
| bmiAMEX-5 | ─ | ─ | |
| bmiBAMMD | + | ─ |
Selected Bcc strains were transformed with shuttle vector pJB3Tc20 (control, no insert), or with pJB3Tc20-derived plasmids equipped with different bmi genes, and tested for susceptibility to BurM1 and BurM2 via spot assay. +, clear halo; T, turbid halo: reduced cell density compared to cell lawn; −, no growth inhibition of the indicator strain.