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. 2015 Nov 27;5:17225. doi: 10.1038/srep17225

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Copyright © 2015, Macmillan Publishers Limited

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(A) HUVECs treated with thrombin for 10 minutes (and subsequently fixed and IF labelled for Vinculin, VE-cadherin and Actin) show radial stress fibre formation and presence of vinculin in the associated FAJs, which indicates high tension on these cell-cell junction complexes. (B–D) HUVECs were virally transduced with expression constructs for α-catenin (tagged by mCherry) as a constitutive cell-cell junction marker and the indicated tension-recruited proteins (tagged by eGFP). Live, wide-field imaging was performed to capture the transition induced by thrombin addition. Still images from the resulting time-lapse series just prior to thrombin treatment and 10 minutes after treatment are shown. Also see Supplemental movie 1–3.