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. 2015 Nov 27;5:17225. doi: 10.1038/srep17225

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Copyright © 2015, Macmillan Publishers Limited

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(A) Representation of VASP, zyxin and TES mutants used for mutational analysis. (B–D) Wide-field microscopy images of FAJs in HUVECs transduced with mutant constructs (B) HUVECs were lentivirally transduced with eGFP-VASP ΔEVH1 or ΔEVH2 mutant constructs, treated with thrombin for 10 minutes to induce FAJ formation and subsequently fixed and IF labeled for α-catenin and F-actin. (C) HUVECs were lentivirally transduced with dsRed-Zyxin 4A, ΔLIM and 4A- ΔLIM mutant constructs, treated with thrombin for 10 minutes to induce FAJ formation and subsequently fixed and IF labeled for α-catenin and F-actin. (D) HUVECs were lentivirally transduced with eGFP-TES mutant constructs containing point mutations in each TES LIM domain, treated with thrombin for 10 minutes to induce FAJ formation and subsequently fixed and IF labeled for α-catenin and F-actin.