Figure 1.

Effect of individual treatment with soy isoflavones or β-carotene on ALP activity in preosteoblast MC3T3-E1 cells. Cells were precultured in α-MEM/10% FBS for 24 h and subsequently cultured in medium with 10% charcoal-treated FBS containing 0.1–10 μM of each of the soy isoflavones or β-carotene for nine days. Data are expressed as means ± SEM (n = 4). Different letters indicate significant differences (p < 0.05). N.S.: not significant.