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. 2015 Nov 27;5:17640. doi: 10.1038/srep17640

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Copyright © 2015, Macmillan Publishers Limited

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(a) The antisense activities of 1 μM NLS-asPNAs assembled on different oligonucleotide scaffolds and of 1 or 10 μM NLS-PNAs without assembly were evaluated in the HBV DNA-integrated cell line HepG2.2.15 for 3 days. (b) HepG2.2.15 cells were treated with NLS-PNAs assembled on the scaffold 12G/C-5A, and the conditions of incubation are indicated on the x-axis. HBeAg in the supernatant was detected for evaluation of antisense activity (n = 3). Comparisons between two groups were carried out with Student’s t-tests. *p < 0.05, **p < 0.01 compared with Con, a**p < 0.01 compared with NLS-PNA/12G/C-5A.