Fig. 2.

Comparison of immunohistochemical staining of GLUT4 in the endometrium from non-PCOS and PCOS patients. Representative paraffin-embedded endometrial sections in the proliferative phase of women without PCOS (A1) and with PCOS (A2), in the secretory phase of women without PCOS (B1) and with PCOS (B2), and in women with hyperplasia without PCOS (C1) and in women with both PCOS and hyperplasia (C2). The same concentration of rabbit IgG instead of the primary and secondary antibodies was used as the negative control (D). Enhanced magnifications are shown in the bottom right corner of A1–D. Immunohistochemistry was performed as described in the Materials and Methods. GLUT4 was found in all endometrial cell types, and specific immunostaining of GLUT4 (DAB-Ni-black) was detected mainly in the membrane and cytoplasm. The images are representative of those observed in numerous sections from multiple endometrial tissues. Scale bar = 100 μm. Ge, glandular epithelial cells; Str, stromal cells.