Fig. 4.

Comparison of immunohistochemical staining for GLUT4 in cultured human endometrial tissues. Endometrial biopsy samples from postmenopausal women were treated with vehicle (A1, A2), hCG (B1, B2), E2 (C1, C2), P4 (D1, D2), or E2 + P4 (E1, E2) for 24 h, fixed in formalin, and embedded in paraffin. The same concentration of rabbit IgG instead of the primary and secondary antibodies was used as the negative control (F). Enhanced magnifications are shown on the right of A1–F. Immunohistochemistry was performed as described in the Materials and Methods. Specific immunostaining of GLUT4 (Ni-black) was detected mainly in the membrane and cytoplasm. Representative images (n = 3 patients) from three independent experiments are shown. Scale bar = 100 μm. hCG, human chorionic gonadotropin; E2, 17β-estradiol; P4, progesterone; Ge, glandular epithelial cells; Str, stromal cells.