Yersinia pseudotuberculosis can enter, replicate and survive up to 1 week within A. castellanii trophozoites. (A) Entry and survival of bacterial strains in trophozoites after pre-culture at 37 or 25°C. Error bars indicate mean ± SD of at least three independent biological replicates. * = P < 0.0001. EC = E.coli DH5α, YPT = Y. pseudotuberculosis IP32953. (B) Transmission electron micrographs of Y. pseudotuberculosis IP32953 localized within A. castellanii trophozoite. (C) Uninfected trophozoite. M = mitochondria, Yp = extracellular Y. pseudotuberculosis, N = nucleus, V = vacuole, P = pseudopodia, Ypi = intracellular Y. pseudotuberculosis. Scale bar representing 2 μm is indicated.