Table 2.
Nucleotide polymorphisms in the PRP-PACAPa, PRP-PACAPb, GHRH and GHRHR genes.
| Region | Number (bp) | Number of Polymorphic Sites | Nucleotide Diversity | Tajima’s D c | Fu’s Fs | dN/dS d | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| π | θw | Fs | p | Stat | p | |||||
| GHRH (KR269823.1) | Synonymous | 101.66 | 2 | 0.00165 | 0.00310 | −0.59938 | ||||
| Silent b | 4431.66 | 112 | 0.00362 | 0.00399 | −0.27736 | |||||
| Non-synonymous | 324.34 | 5 | 0.00210 | 0.00243 | −0.24643 | |||||
| Total gene a | 4756 | 117 | 0.00352 | 0.00388 | −0.28353 | −126.856 | 0.000 | 0.250 | 0.803 | |
| PRP-PACAPa (KR269822.1) | Synonymous | 127.00 | 0 | 0.00000 | 0.00000 | NA | ||||
| Silent b | 4457.00 | 81 | 0.00318 | 0.00287 | 0.00267 | |||||
| Non-synonymous | 395.00 | 1 | 0.00006 | 0.00040 | 0.00267 | |||||
| Total gene a | 4852 | 82 | 0.00292 | 0.00267 | 0.00267 | −134.514 | 0.000 | 1.029 | 0.305 | |
| PRP-PACAPb (KR269834.1) | Synonymous | 134.82 | 0 | 0.00000 | 0.00000 | NA | ||||
| Silent b | 5084.82 | 53 | 0.00107 | 0.00164 | −1.01201 | |||||
| Non-synonymous | 465.18 | 1 | 0.00016 | 0.00034 | −0.50730 | |||||
| Total gene a | 5550 | 54 | 0.00099 | 0.00154 | −1.02345 | −73.275 | 0.000 | 0.000 | 1.000 | |
| GHRHR (KR269832.1) | Synonymous | 82.19 | 2 | 0.00266 | 0.00384 | −0.39137 | ||||
| Silent b | 4009.19 | 79 | 0.00285 | 0.00311 | −0.24244 | |||||
| Non-synonymous | 265.81 | 6 | 0.00311 | 0.00356 | −0.24054 | |||||
| Total gene a | 4280 | 85 | 0.00287 | 0.00313 | −0.25251 | −46.197 | 0.000 | 0.202 | 0.840 | |
a The total number of fragment sites was assessed; b Silent substitutions were considered in coding and noncoding regions; c All Tajima’s D values were not significant (p > 0.10); d The ratio of the rates of non-synonymous and synonymous substitution.