Table 5.
Primers of the four genes (four fragments).
ID | Gene | Forward 5′ to 3′ | Reverse 5′ to 3′ | Amplicon Length 3 | GenBank Accession Number or Predicted (mRNA) |
---|---|---|---|---|---|
KR269823.1 | GHRH 1 | TGTGGGAGTGACTGGGAGC | GAGACACGCACAATACCAG | 4756 | GU966634.1 |
KR269822.1 | PRP-PACAPa 1 | GATGCACTCCAATGGGAC | GAGTCGTCTGCACAGATG | 4852 | predicted |
KR269834.1 | PRP-PACAPb 1 | GACTGCTCCTTCTTGGTTAA | TTTGTCTCCTGCTCTTCCT | 5550 | AY869693.1 |
KR269832.1 | GHRHR 2 | GCTTTGTCGAGCCTGCACC | CAACACAGCAACAACCAGC | 4280 | GU966635.1 |
1 The amplicon includes the entire CDS; 2 The amplicon includes part of the gene; 3 The number excludes the length of the two primers at the end of the amplicon.