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. 2015 Nov 3;16(11):26291–26302. doi: 10.3390/ijms161125948

Figure 4.

Figure 4

Notch1 inhibition decreases proliferation in adult and old keratinocytes. (A) Adult and old cells were analyzed 24 h after 0 and 50 µM DAPT treatment, and levels of N1ICD, survivin, and K10 were determined by Western blotting analysis. β-actin was used as loading control. Bar graphs show the average densitometry values normalized to β-actin; (B) Cell cycle was evaluated in adult and old keratinocytes 24 h after 0 and 50 µM DAPT treatment by flow cytometry; (C) Relative percentile quantification of adult and old cell proliferation after 50 µM DAPT treatment by MTT assay. Normalization was calculated as compared to control. * 0.01 < p < 0.05; ** p < 0.01.