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. 2015 Nov 4;16(11):26395–26405. doi: 10.3390/ijms161125965

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ser284 and Ser292 are RMI1 mitotic phosphorylation sites. The indicated RMI1 mutants were transiently transfected into 293T cells followed by nocodazole treatment or not for 16 h. Ectopic expression of wild type (WT), T270V, S284A or S292A (A); or S284A/S292A, T270V mutation of RMI1 (238–625) fragment (B); (C) Ectopic expression of full-length wild-type RMI1 or S284A/S292A mutant. Nocodazole treatment and phosphatase treatment were applied as indicated. Quantified relative RMI1 protein levels are shown under the corresponding blots.