Figure 5.

Binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to S23906-1 DNA adducts. (A) Increased selectivity for adduct recognition in the course of binding site selection is visualized upon migration of the radiolabeled mixture of DNA with GAPDH (5 μg) on a native 6% polyacrylamide gel. “0” and “G” refer to the absence or presence of GAPDH proteins; (B) Determination of the preferential binding site upon alignment of the amplified sequences; (C) EMSAs evidence a differential complexation efficiency of GAPDH on S23906-1 alkylated (labeled “with S23906-1”) or unalkylated (labeled “without S23906-1”) using various DNA sequences from [176] and comparison with the reference DNA Smad-SBE (Smad).