Table 1.
Sequences of the bound oligonucleotides. The name and sequences of oligonucleotides correspond to that that formed a GAPDH/DNA complex formed a revealed using Transignal membranes presented in Figure 7. In grey: sequences that contains the 5′-GGT(G/T)(G/T) site. Underlined: potential GAPDH binding sites from comparison with sequences obtained from SELEX. “+” and “++” refers to strong and very strong binding of GAPDH to DNA at the indicated sequence.
| TranSignal Membrane | Transcription Factor | GAPDH Binding | Sequence for TranSignal Oligonucleotides Containing Transcription Factor Consensus Sites |
|---|---|---|---|
| I | ARE | + | GTCTGGTACAGGGTGTTCTTTTT |
| CDP | + | TCAGAAATTGGCTAATAATCATTGGG | |
| E2F1 | ++ | ATTTAAGTTTCGCGCCCTTTCTCAA | |
| EGR(1) | + | GGATCCAGCGGGGGCGAGCGGGGGCCA | |
| GAS/ISRE | + | CGAAGTACTTTCAGTTTCATATTACTCTACAA | |
| MEF-1 | ++ | TCAGGCAGCAGGTGTTGGGGGGAT | |
| SIE | + | GTGCATTTCCCGTAAATCTTGTCTACA | |
| II | c-Rel | + | GGGGATTTCCGGGGATTTCCGGGGATTTCC |
| E4F/ATF | + | GGCTGACGTCACTGGGCTGACGTCACTG | |
| AFXH (FOXO4) | + | GTTGTTTATGGTTGTTTATGGTTGTTTATG | |
| Freac2-2 | + | TTGTTTTGTTGTTTTGTTGTTTTG | |
| HFH3 | + | GGGTGTTTGTTTAGGGTGTTTGTTTA | |
| MEF-2 (2) | ++ | GCTATTTTTAACGAGGGCTATTTTTAACGAGG | |
| PARP | ++ | ATGGGAGGGGCAATGGGAGGGGCA | |
| PAX3 | + | GATCCTGAGTCTAATTGGATCCTGAGTCTAATTG | |
| REBB2 | ++ | TGGAAATGGCGGGGGATGGTGGGGGACCGGATC | |
| RSRFC4 | + | GGTCTATTTATAGCTTGGTCTATTTATAGCTT | |
| ZIC | + | CATAGTTTCTAAAAGAGGAGGAGGTAGTTCTAG | |
| III | ADD-1 | + | TCCTAGTGTGAGCGGCCCT |
| HMG | + | CGATCTGGAACTCCGGGAATTTCCCTGGCCC | |
| HOXD-8/9/10 | + | GCGGCAGTTTTATTGTTTTATTCGC | |
| MAZ | + | GGGTTGGGGAAGTATTAGGAGGGGAGGGTT | |
| NF-4FA | + | CTCCTTTCTTTGAAGCTCCTTTCTTTGAAG | |
| TCE | ++ | GCAGAGGGCGTGGGGGAAAAGAA | |
| IV | AP-4 | + | TCAGCGCGGGTCAGCGCGGGATTC |
| ApoA1Prom | ++ | CCCTGCAAGAGCTGGCTGCTTAGAGACTGCGAGAAGGAG | |
| c-Myb (2) | + | GGACCAGGGGGTCTAGGAG | |
| CYP1A1 | ++ | GTAAGGGGGCAGAGGTCGGG | |
| EGR1 (2) | ++ | CCTCCCCCCGCCTTGCCCGGGGTTGTGG | |
| kBF-alpha | + | GGCGTTTTCGTTTTTACCCGGC | |
| LXRE-1 | + | GCTGAGGTTACTGCTGGTCATTCAAGCT | |
| MASH-1 | + | GGCTCAGGCAGCAGGTGTTGGG | |
| MBP-1 (1) | + | GTGGGAAATTCCGTGGGAAATTCC | |
| V | MDBP (1) | ++ | CTATTGGCGTTACTATGGGAACATA |
| MDBP (2) | + | GGCCATTACCTGGTGATATTACCTGGTGATGC | |
| Myb (2) | + | GCCCAGTTGTTAGCCCAGTTGTTA | |
| NCAM-BP | ++ | GCTCTGCATTTTCTTTTGGCC | |
| NF-Atp | + | TTGCATTTTCCATGGTTGCATTTTCCATGG | |
| NF-E6/CP1 | + | ACTGAGTCATGAGTCATGGTTGGCCC | |
| ODC | + | TGCGTCTCCATGACGGTCTCCATGACGAC | |
| PAX-1 | + | CACCGTTCCGCTCTAGATATCTC | |
| PBGD BP | + | TCAGTGTCCTGGAGTGTCCTGGTTACT | |
| Pit-1 (1) | + | CTAAATTATCCATTTATCCATTAGCAC | |
| PU.1 | + | AGAAAAGGAGAAGTAGGAGGC | |
| Snail | + | TGTGAACAGGTGCTTGTGAACAGGTGCT | |
| Thy-1 BP | + | GATCAGGGGTGGCAGGGGTGGAAT |