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. 2015 Nov 5;16(11):26599–26607. doi: 10.3390/ijms161125972

Figure 2.

Figure 2

Compound B inhibits RANKL-induced osteoclastogenesis. (A) Chemical structure of compound B; (B) mouse BMMs cultured in a 96-well plate in the presence of RANKL (100 ng/mL) and M-CSF (10 ng/mL) with or without compound B for five days were fixed with 4% paraformaldehyde and stained for TRACP activity. Quantitative analysis shows the mean number of TRACP-positive multinucleated cells (MNC). (*** p < 0.001 compared to RANKL treated control, n = 3); and (C) representative light microscope images showing the effect of compound B on RANKL-induced osteoclast formation. (Mag = 20×, scale bar = 100 µm).