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Cell viability assay. Live/Dead Viability/Cytotoxicity Kit for mammalian cells (Invitrogen, Carlsbad, CA, USA). (A) One-day cell culture; (B) three-day cell culture; (C) seven-day cell culture; and (D) control scaffold without cells to exclude natural collagen fluorescence. Live cells are green (objective, ×4). Scale bars represent 200 μm.
