Table 2.
Effect of scavengers of reactive oxygen species on flavonoid-induced cellular DNA breakage in permeabilized cells. * p < 0.05, compared with control (flavonoid only) cells. Data represent ±SEM of three independent experiments.
| Treatment | Intact Cells | Permeabilized Cells | ||
|---|---|---|---|---|
| Comet Tail Length % | Inhibition (µM) | Comet Tail Length % | Inhibition (µM) | |
| Control | 3.156 ± 0.17 | - | 3.25 ± 0.16 | - |
| Myricetin (100 µM) | 25.03 ± 1.25 | - | 31.56 ± 1.57 | - |
| +SOD (100 µg/mL) | 11.26 ± 0.56 * | 55.0 | 13.28 ± 0.76 * | 57.9 |
| +Catalase (100 µg/mL) | 12.46 ± 0.62 * | 50.2 | 15.05 ± 0.80 * | 52.3 |
| +Thiourea (1 mM) | 14.08 ± 0.70 * | 43.7 | 16.86 ± 0.84 * | 46.5 |
| Fisetin (100 µM) | 22.96 ± 1.14 | - | 27.04 ± 1.35 | - |
| +SOD (100 µg/mL) | 12.20 ± 0.61 * | 46.8 | 14.24 ± 0.71 * | 47.3 |
| +Catalase (100 µg/mL) | 12.76 ± 0.63 * | 44.4 | 14.64 ± 0.73 * | 45.8 |
| +Thiourea (1 mM) | 13.04 ± 0.65 * | 43.2 | 15.05 ± 0.75 * | 44.3 |
| Quercetin (100 µM) | 20.32 ± 1.01 | - | 24.54 ± 1.22 | - |
| +SOD (100 µg/mL) | 10.46 ± 0.52 * | 48.5 | 11.23 ± 0.56 * | 54.2 |
| +Catalase (100 µg/mL) | 11.06 ± 0.55 * | 45.5 | 13.42 ± 0.67 * | 45.3 |
| +Thiourea (1 mM) | 14.80 ± 0.74 * | 27.1 | 14.95 ± 0.74 * | 39.07 |
| Kaempferol (100 µM) | 17.63 ± 0.88 | - | 21.54 ± 1.07 | - |
| +SOD (100 µg/mL) | 9.50 ± 0.47 * | 46.1 | 10.36 ± 0.51 * | 51.9 |
| +Catalase (100 µg/mL) | 10.44 ± 0.52* | 40.7 | 10.45 ± 0.52 * | 51.4 |
| +Thiourea (1 mM) | 11.05 ± 0.55 * | 37.3 | 12.45 ± 0.62 * | 42.2 |
| Galangin (100 µM) | 14.40 ± 0.72 | - | 18.44 ± 0.92 | - |
| +SOD (100 µg/mL) | 8.55 ± 0.32 * | 40.6 | 9.65 ± 0.63 * | 47.6 |
| +Catalase (100 µg/mL) | 9.40 ± 0.37 * | 34.7 | 11.32 ± 0.59 * | 38.6 |
| +Thiourea (1 mM) | 10.15 ± 0.50* | 29.5 | 10.55 ± 0.53 * | 42.7 |