Figure 1.

Berberine sulfate inhibits RANKL-induced osteoclastogenesis in BMM cells. (A) Chemical structure of berberine sulfate. The molecular weight of berberine sulfate is 433.43; (B) The 96 well-plate showing the effects of different concentration of berberine sulfate on the BMMs derived osteoclast-like cell formation. BMM cells (6 × 10³ cell/well) were cultured in the presence of M-CSF and GST-rRANKL (50 ng/mL) with or without different concentration of berberine sulfate for five days. Then, the cells were stained for TRAcP. “−“ means RANKL untreated; and“+” means RANKL treated; (C) Enlarged images of B (scale bar: 100 µm); (D) Osteoclast cell counts showing TRAcP-positive multinucleated cells. (n = 3); (E) Effect of berberine sulfate on the viability of BMM cells as measured by MTS assay. (n = 3). *** p < 0.001 (versus RANKL-treated control).