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Berberine sulfate suppresses osteoclast function. (A) Representative images of osteoclastic resorption and TRAcP staining on hydroxyapatite coated surfaces (Scale bars, 500 µm); (B) The effect of berberine sulfate on the number of TRAcP positive multinucleated cells (Nuclei ≥ 3, counted as osteoclasts); (C) Percentage of the area of hydroxyapatite surface resorbed per osteoclast. * p < 0.05, *** p < 0.001 relative to untreated controls.
