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Effect of berberine sulfate on mRNA levels of osteoclast-associated genes. Real time-PCR analysis was performed to examine osteoclast-specific gene expression (Ctsk, V-ATPase d2, NFATc1, and TRAcP), and results were normalized to the expression of GAPDH. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with untreated control.
