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. 2015 Nov 13;16(11):27087–27096. doi: 10.3390/ijms161125998

Figure 5.

Figure 5

Berberine sulfate suppressed RANKL-induced NFAT activity. (A) RAW264.7 cells were stably transfected with an NFAT-dependent transcriptional luciferase reporter construct and then pretreated with indicated concentration of berberine sulfate for 1 h, prior to the addition of RANKL (50 ng/mL) for 24 h. The cells were harvested, and the luciferase activity was measured (n = 6). “−“ means RANKL untreated; “+” means RANKL treated. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with untreated control; (B) BMM cells were pretreated with berberine sulfate for 1 h, followed by RANKL (100 ng/mL) stimulation for indicated times. The cell lysates were probed for NFATc1 protein levels using Western blot analysis.