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. 2015 Nov 12;16(11):27058–27071. doi: 10.3390/ijms161126001

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gating strategy for detection and sorting various hematopoietic progenitor cells in the bone marrow by FACS. P1 is gated on the viable bone marrow cells from all events; P2 is gated on the P1-gated viable cells; P3 is gated on the P2-gated cells to remove doublets ; P4 is gated on the P3-gated cells to remove doublets; P5 is gated on Lin⁻c cells from P4-gated cells; P6 is gated on Lin⁻c-Kit⁺ cells from P5-gated cells.