Danu alters the expression of pro-apoptotic and anti-apoptotic proteins in C13 and A2780cp cells. C13 and A2780cp cells were incubated with Danu 0.01, 0.1, and 0.5 μM for 48 h and the protein samples were subject to Western blotting assay. (A) Representative blots of B-cell lymphoma-extra large (Bcl-xl), B-cell lymphoma 2 (Bcl-2), Bcl-2-like protein 4/Bcl-2-associated X protein (Bax), p53 up-regulated modulator of apoptosis (PUMA), cytochrome c, c-caspase 9, and c-caspase 3 in C13 and A2780cp cells and (B) bar graphs showing relative expression level of Bcl-xl, Bcl-2, Bax, PUMA, cytochrome c, c-caspase 9, and c-caspase 3 in C13 and A2780cp cells. β-actin was used as the internal control. Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 by one-way analysis of variance.