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. 2015 Nov 13;16(11):27228–27251. doi: 10.3390/ijms161126018

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Danu induces autophagy in C13 and A2780cp cells over a 72-h treatment period determined by flow cytometry. C13 and A2780cp cells were incubated with Danu 0.5 μM for 4, 8, 12, 24, 48, and 72 h and the samples were subject to flow cytometry. (A) Flow cytometric plots showing autophagy of C13 and A2780cp cells and (B) bar graphs showing the percentage of autophagic C13 and A2780cp cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001 by one-way analysis of variance.