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. 2015 Nov 16;16(11):27391–27400. doi: 10.3390/ijms161126025

Figure 2.

Figure 2

Heparin affinity chromatography of cationic peptide hormones. (A) Chromatograms of PACAP38 elution with increasing NaCl concentration on a sepharose heparin column connected to a FPLC Aktapure system. The NaCl gradient is shown as dashed line; (B) Relative affinities of peptide hormones for heparin. The histogram indicates the concentration of NaCl required for eluting a given peptide from the heparin sepharose resin; (A,B) For all experiments: injection of 250 μg of peptides in phosphate buffer (20 mM, pH 7.4) and flow rate of 0.5 mL/min.