Figure 5.

Effects of Bay11-7082 on leptin-regulated cPLA₂-α expression and p65 phosphorylation. Serum-starved A549 cells were pretreated with different concentrations of Bay11-7082, OB-R (2 µg/mL), U0126 (10 µM), SB202190 (10 µM), SP600125 (10 µM), or Bay11-7082 (10 µM) for 1 h. The cells were then stimulated by 1 µg/mL of leptin for the indicated time intervals. At the end of incubation, the cells were harvested and cell lysates were extracted. (A) Expression of cPLA₂-α and (B) Ser276 phosphorylation of p65 were detected using Western blot with specific antibodies. The cell membranes were stripped and reprobed with the anti-GAPDH antibody as internal controls. The data are expressed as mean ± SEM of five independent experiments (n = 5). & p < 0.05 as compared with the cells exposed to the vehicle alone; # p < 0.01 as compared with the cells exposed to leptin.