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. 2015 Nov 20;16(11):27781–27795. doi: 10.3390/ijms161126062

Figure 1.

Figure 1

Figure 1

miR-218 is embedded within CpG islands. (A) Schematic illustration of the miR-218-1 gene embedded in the intron of SLIT2 at 4p15.31, whose promoter region is embedded within a CpG island (black box). The transcription start site is designated as +1. The region −760 to −344 upstream of TSS was amplified for bisulfite sequencing, while −676 to −518 was amplified for methylation-specific PCR; and (B) schematic illustration of the miR-218-2 gene embedded in the intron of SLIT3 at 5q35.1, whose promoter region is also embedded within a CpG island (black box). The region −407 to −14 upstream of TSS was amplified for bisulfite sequencing, while −285 to −47 was amplified for methylation specific PCR. Vertical bars represent CpG dinucleotides. Arrows indicate the direction of gene transcription. TSS: transcription start site; BSP: bisulfite sequencing analysis; MSP: methylation specific PCR.