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. 2015 Nov 20;16(11):27781–27795. doi: 10.3390/ijms161126062

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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miR-218 suppresses cell proliferation. (A) MiR-218 expression in EC109 cells increased significantly when treated with 30 nM miR-218 mimic compared with the negative control (NC); (B) Cells labeled in red after reaction of EdU (5-ethynyl-2′-deoxyuridine) and Apollo represent proliferative cells. Cell nuclei stained with Hoechst 33342 (blue) represent the total cells. The images are obtained by fluorescence microscope; (C) The percentage of proliferating cells was significantly increased (p < 0.001) in EC109 cells treated with miR-218 mimic than those in control cells.