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. 2015 Nov 20;16(11):27781–27795. doi: 10.3390/ijms161126062

Figure 8.

Figure 8

miR-218 targets 3′UTR of ROBO1. (A) The putative binding sites of miR-218 targeted the 3′UTR of ROBO1; (B) MiR-218 significantly down-regulated luciferase activity of wild type 3′UTR of ROBO1 which suggests that miR-218 directly targets the 3′UTR of ROBO1 (p < 0.001), but did not affect luciferase activity of mutant 3′UTR of ROBO1. WT: wild type 3′UTR of ROBO1; Mut: mutant 3′UTR of ROBO1; (C) Expression of ROBO1 mRNA was detected by qPCR in EC109 treated with miR-218 mimic and negative control; (D) ROBO1 was detected by western blot in EC109 treated with miR-218 mimic and negative control. miR-218 significantly down-regulated ROBO1 at both mRNA and protein levels. NC: negative control.