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ATP-DnaA requires additional ATP mix to unwind oriC. (Top) supercoiled oriC plasmid was incubated with the indicated concentrations of ATP-DnaA or ADP-DnaA in the absence or presence of 5 mM ATP or 5 mM ADP. After P1 endonuclease digestion, DNA species were resolved by electrophoresis. The supercoiled (sc), nicked, and linear forms are marked; (Bottom) the linear form generated by P1 digestion in the presence or absence of 5 mM ATP and 5 mM ADP respectively were quantitated.
