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Multi-enzyme system for C–C bond formation catalyzed by DHAP-dependent aldolases, based in the in situ phosphorylation of DHA catalyzed by DHAK from Citrobacter freundii (C. freundii). DHA: Dihydroxyacetone; DHAK: dihydroxyacetone kinase; AK: acetate kinase; RAMA: rabbit muscle aldolase; DHAP: dihydroxyacetone phosphate.
