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. 2015 Nov 26;13:28. doi: 10.1186/s12953-015-0085-2

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© Ooi et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overlay of Cy3 and Cy5 gel images of a 2-dimensional DIGE gel. DIGE gels were produced for spot analysis using 13 cm IEF strips of pH 4–7 with 20-kVh focusing. Mesocarp protein from high yielding palms was labeled with Cy3 whilst mesocarp protein from low yielding palms with Cy5. A total of nine DIGE gels were produced for analysis from three biological replicates of 12, 16 and 18 WAP fruit. Numbers in the gel indicate the geographical position of protein spots in the map